(from south to north)
The towns and villages served by the route are listed below.
Train services on the line are operated by several train operating companies.
- First Capital Connect operate through services to London King's Cross via Cambridge and Stevenage. These services operate non-stop between London and Cambridge for most of the day, as part of the half-hourly "Cambridge Cruiser" service. One train an hour is extended beyond Cambridge to serve all stations to King's Lynn. These services usually use Class 365 electrical multiple units, although Class 317 units are used occasionally.
- There are three "named" units, the first unit (365518) is named "The Fenman" which was previously a "named train" that used to operate on this line, consisting of a locomotive-hauled Inter-City service. The second unit (365531) has been named "Nelson's County - Norfolk", this unit has had a special livery applied to the outside which shows various scenes of West Norfolk. Wagns last naming was unit 365 527, this was named on 10th March 2006 "FLUA 21" after the 21st Anniversary of the Fen Line Users Association
- 'one' operate some direct services from London Liverpool Street to King's Lynn via the West Anglia Main Line and Fen Line. These services operate only during the morning and evening peaks, and use Class 317/6 units (the refurbished ones with fewer, higher seats).
- The section between Cambridge and Ely is also used non-stop by Central Trains services to Stansted Airport, and by 'One' services between Cambridge and Norwich via the Breckland Line.
The line is electrified at 25 kV AC, using overhead wires. Electric services started to King's Lynn from 28th August 1992. It has an active User Association which is called the Fen Line Users Association or FLUA